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NAPstars specifically respond to dynamic NADP redox changes in vitro To further test the dynamic and specific responsiveness of NAPstars to changes in NADP redox status, we used recombinant NAPstar probes to monitor NADP dynamics in enzyme assays in vitro (Supplementary Fig

Briefly, 0.1 g leaves were extracted with 10 mL of 10% trichloroacetic acid (TCA) and then centrifuged at 10,000g for 20 min

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For intermediate precision of the method, the intraday and inter-day analysis was done, i.e., repeated the same concentrations six times a day at a gap of one hour each ( n = 6) and on 6 successive days, respectively