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IGF-1 LR3 has been used extensively in this context precisely because its extended half-life allows sustained receptor activation in cell culture and in vivo models without the impractical dosing frequency that native IGF-1 would require

Cell lines harboring a PIK3CA -activating mutation or a PTEN -inactivating mutation showed ferroptosis resistance mediated via activation of the PI3K/AKT/mTOR pathway, which further upregulates SREBP1 101

J., Beckmen, K

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Formalin-fixed, paraffin-embedded tissue sections were stained using a TUNEL Apoptosis Detection Kit (Yeasen) according to the manufacturers instructions