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Confocal images of organoids and thick tissue sections were acquired on an inverted single-photon point scanning confocal microscope (Zeiss Cell Discoverer 7 with LSM 900 and Airyscan 2) using a Zeiss APOCHROMAT 20/0.95-NA Autocorr air objective, with a tube lens of 0.5 or 1, and a voxel size of 0.4 0.4 0.5 m or 0.5 0.5 0.5 m for organoids and 0.3 0.3 0.3 m for thick tissue sections

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The animal models behave normally without signs for pain, stress, or discomfort

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PCOS is a risk factor for cardiometabolic diseases, gestational diabetes, and pregnancy complications