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For that reason, women who are pregnant or planning a pregnancy shouldnt take excessive doses of vitamin A

Ascorbic acid (1 mL) was added to a final concentration of 300 M

The cells were then lysed with 2% digitonin in PBS and the protein concentration was determined using the BCA assay kit

Principal component analysis (PCA) indicated that Day 30 iHLOs exhibited a protein expression pattern more closely aligned with human liver tissue than HepaRG and HepG2 cells (Fig