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For reconstituting a 50mg vial, a 3mL or 5mL syringe is typically best for accurately measuring the larger volume of BAC water

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Notably, Atg11 focus assembly occurs independently of downstream factors (Atg9 and Vac8), highlighting its role as an LLPS-driven early platform for the recruitment of the autophagy machinery, which is distinct from later maturation steps

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