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Table 1) with glioblastoma after approval from the Institutional Review Board at Seoul National University Hospital (IRB No

doi:10.1016/0014-5793(75)80279-1

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The reconstituted nanodiscs sample was further purified using immobilized metal ion affinity and then SEC to remove nanodiscs without target protein, the latter in the presence of 20 mM Tris pH = 7.5, 100 mM NaCl

To remove excess DTT, 0.15 ml of 0.5% N -ethylmaleimide was added after incubation for 10 min at room temperature