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Finally, staining was performed with 3,3-diaminobenzidine (DAB, Sigma, D12384) substrate and counterstained with hematoxylin for nuclear differentiation

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In mature astrocytes differentiated from human induced pluripotent stem cells (iPSC)-derived astrocyte progenitors, ketamine can exert rapid antidepressant effects through the activation of amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) glutamate receptors, and estrogen enhances this effect of ketamine by increasing the gene expression of AMPA receptor subunits