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A neuronal lactate uptake inhibitor slows recovery of extracellular ion concentration changes in the hippocampal CA3 region by affecting energy metabolism

Hematin (20 M) was dissolved in varying concentrations of GSH (05 mM) and the difference in absorption between 618 nm and 655 nm was plotted against GSH concentration, leading to the determination of the affinity constant, Ka, 5.0 10 4 M 1

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Anesthesia and euthanasia protocol Each rat was anesthetized using 23% isoflurane in oxygen