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Database search criteria largely followed the default settings for directDIA including: tryptic with two missed cleavages, carbamidomethylation of cysteine, and oxidation of methionine and precursor Q-value (FDR) cut-off of 0.01

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Pyroptosis levels strongly indicate that the thermal tumor immune microenvironment has a high presence of CD8 + T cells and other T cell subtypes, as well as activation of P53 pathway, DNA repair, KRAS signaling, epithelial-mesenchymal transformation (EMT), IL6 JAK STAT3 signaling, IL2 STAT5 signaling, PI3K signaling, AKT signaling, mTOR signaling, and oncogenic pathways are enriched in the pyroptosis-Hi subgroup of cancer (132)