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In the down state, RBD angles are close to the central cavity of the trimer to shield the receptor-binding regions, while in the up state, the RBD undergoes hinge-like conformational movement, exposing its determinant regions to recognize the human angiotensin-converting enzyme 2 (hACE2) receptor on the host cellular membrane, the state of which is considered to be less stable than in the down state

A detailed review of the TBA test was conducted by Guillen-Sans and Guzman-Chozas in 1998

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Using duct tape to fix a broken heart is not an easy process