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Cheng S-E, Luo S-F, Jou M-J, Lin C-C, Kou YR, Lee I-T, Hsieh H-L, Yang C-M (2009) Cigarette smoke extract induces cytosolic phospholipase A2 expression via NADPH oxidase, MAPKs, AP-1, and NF-B in human tracheal smooth muscle cells

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Thereafter, the plate was washed three times and a 100-L of detection reagent B working solution was added to each well, and the plate was incubated for 30 min at 37 C