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Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal

These studies suggest that subcellular compartmentalization of GSH, rather than total cellular GSH abundance alone, is a critical determinant of apoptotic sensitivity

Cell viability assay For cell viability assay, the Cell Counting Kit-8 (CCK-8) (Topscience, Shanghai, China) was prepared to assess cell viability following the manufacturers instructions

Methods Molecul Biol (Clifton, NJ)

The relative expression level of the target gene was calculated using the 2 CT method, and each group of data was repeated with at least three independent samples