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LIPUS treatment can improve the regeneration of nerve fibers reaching the target muscles, so it can prevent the irreversible muscle atrophy

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After drug treatments, the cells were fixed with 4% paraformaldehyde for 20 min and infiltrated with 0.2% Triton X-100 for 15 min, followed by blocking in donkey serum for 30 min and incubating overnight at 4C with primary antibody (GPX4, rabbit, ab125066, 1:200, Abcam) in PBS

This leakage further activates microglia towards the M1 phenotype, promoting release of neurotoxic agents, including TNF- and IL-6 (50)

(2019) mTOR-dependent upregulation of xCT blocks melanin synthesis and promotes tumorigenesis