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NIH/3T3 cells were seeded on 6-well plates (2 10 4 cells per well) and cultured for 24 h

With annual costs of over EUR 30 billion per year in Europe and USD 5.3 billion in the USA and treatments that are trying to modulate the microbiome or innate immune response, targeting itching or inhibiting Janus kinases, AD affects up to 20% of children and up to 10% of adults worldwide [80]

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In that study, alpinetin decreased the expression levels of IL-1, IL-6 and TNF- ( Supplementary Table 1 ) in LPS-treated RAW 264.7 macrophages in vitro and in the in vivo LPS-induced acute lung injury murine model (107) by disrupting ERK/p38MAPK signaling (193)

39 Bruner RH, Kinkead ER, O'Neill TP, et al