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Briefly, RAFLS (6000 cells/well) were cultured in 96-well plates before treated by epalrestat

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FMT in animal models has been found to reduce the abundance of the Akkermansia genus (in phylum Verrucomicrobia) and increase the abundance of the Prevotella genus (in phylum Bacteroidetes) in gut microbiota (Tankou et al., 2018b), which is in line with findings of decreased gut Akkermansia after probiotic interventions and increased gut Prevotella after first-line disease-modifying treatments and time-restricted eating in MS patients (Jangi et al., 2016