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Vpr induces oxidative stress by stabilizing p53 protein, increasing transforming growth factor-beta protein levels and ROS production, and suppressing antioxidant enzymes 118,119,120

In addition, SOD was measured using specific colorimetric assay kits (Arbor Assays Company, 1514 Eisenhower Place Ann Arbor, Michigan 48108 USA, Catalog Number K028-H1), while GPx was measured using particular colorimetric assay kits (BioAssay Systems Company, Hayward, CA 94545, USA, Catalog Number: EGPX-100) following the manufacturers instructions for each test