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Afterwards, the samples were centrifuged for 30 min at 17,000 g

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Arabidopsis vtc2-1 accumulates only 10 to 30% of the wildtype Asc level, which results from a mutation in the GDP-l-galactose phosphorylase 1 gene, the enzyme catalyzing an intermediate step in Asc synthesis (Mller-Moul et al