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In brief, plated cells were incubated for 30 min at 37 C, 5% CO 2 in the staining solution (HBSS containing 1 g ml 1 Hoechst 33342 (62247, Invitrogen, Thermo Fisher Scientific) and either 5 M CellROXTM, 1 M FerroOrange or 1 M BODIPY 581/591 C11)

Statistical analysis Results are presented as meanS.E.M

In addition, the ratios of C5DC to C5-OH and C8 to C2 were significantly positively associated with fracture, but the ratios of C4 to C8, C5 to C0, C5 to C2, C5 to C3, C5-OH to C8, C5-OH to C0, C3DC to C10, and C5DC to C8 were negatively associated with fracture

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381 Similarly, in patients with locally advanced ESCC undergoing neoadjuvant chemotherapy combined with immunotherapy, Wu et al