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The day of the experiment, cells were treated with test compounds for the indicated times, then harvested by trypsinization, resuspended in 500 L Hanks Balanced Salt Solution (HBSS, Gibco, Invitrogen Corp., Carlsbad, Calif.) containing either H 2 DCFDA (25 M), C11-BODIPY(581/591) (2 M) or MitoSOX (5 M) (all from Molecular Probes, Invitrogen) and incubated for 10 minutes at 37 C

However, the characteristics of each component and their interrelationships remain to be deeply understood

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The solution is simple: just alternate when you use them

Impact of weekly iron folic acid supplementation with and without vitamin B12 on anaemic adolescent girls: A randomised clinical trial