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Photostability evaluation To test the photostability in buffer and cell culture media, PLGA nanoparticles encapsulating DDA-GSH-AuNCs in water (2 mg/mL) was mixed with water (control), PBS or DMEM that contains 10% FBS (final concentration of PLGA nanoparticles is 0.5 mg/mL and AuNCs 4 g/mL as gold)

Previously unreported effects of RSL3, such as degradation of mTOR signaling pathway protein, p70S6K, and reduction of p-NPM1 levels were identified in the present study and warrant further investigation

doi: 10.1111/papr.12516

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Researchers favor this approach for its accessibility, well-documented protocols, and compatibility with diverse biological sample types including cell lysates and tissue homogenates