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Preparation of DHA-N@M To investigate the optimal ratio, RCM and DHA-SNO were mixed in PBS at different weight ratios and incubated for 0.5 h in a 37 C water bath to form DHA-SNO-inserted RCM nanoreactors (DHA-N@M), followed by wash with PBS to remove free DHA-SNO

A validation of these pockets observed in FBDD by XRD (with notably exception of evident functional sites that can be derived from prior enzymological knowledge) with respect to druggability usually requires follow-up experiments as typically carried out in F2L and medicinal chemistry studies 90

Storage: Keep refrigerated between 28C

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With cofactor support: Combined with vitamin C, NAC, and B6 to support glutathione metabolism