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The HRP reaction was terminated by addition of 1 M HCl and the 450 nM (HRP) or 405 nm (AP) absorption values was recorded using a Sunrise Spectrophotometer (TECAN)

J Am Heart Assoc Cardiovasc Cerebrovasc Dis 9(18):17533

A knowledgeable surgeon can help you understand the current evidence, potential risks, and evidence-based alternatives such as PRP (platelet-rich plasma) or other biologic treatments that have established clinical support.