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Notably, addition of hemin or pyruvate, a source for NAD+ did not impact these growth phenotypes, suggesting that amelioration of heme defect or respiration inhibition is not sufficient to overcome anti-proliferative effects of mitochondrial GSH depletion (Extended Data Fig

Modulation of oxidative stress, inflammation, and atherosclerosis by lipoprotein-associated phospholipase A2

Then, the PCR reactions were conducted using a previously described protocol 41

Calcein AM for cell viability analysis by flow cytometry Cells were seeded in 96 well plates at 3.0e5 cells/well in 100 l of RPMI-1640 with 10% FBS

Caverzan et al., 2014