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The total cell lysate was cleared by centrifugation and supplemented with 25 L per liter culture OD 600 of BioLock avidin (IBA Lifesciences, Gttingen, Germany) to mask D-biotin from the culture medium and/or endogenous biotinylated proteins from the host cell, followed by sterile-filtration

Substrate (e.g., glucose) consumption and oxygen consumption continue, and will actually increase, because the protons are not being pushed out against a gradient and just flow right back in without doing any work to make ATP

J Neurochem 97:1634-1658 Ballatori N, Krance SM, Notenboom S, Shi S, Tieu K, Hammond CL (2009) Glutathione dysregulation and the etiology and progression of human diseases

Grover, A.) 113133 (Springer, Singapore, 2017)

A global survey of the role of ultraviolet radiation and hormonal influences in the development of melasma