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Western blot analysis Total protein extracts from liver tissues and HepG2 cells were prepared using RIPA buffer containing 50 mM Tris-HCl, 150 mM NaCl, 5 mM MgCl2, 2 mM EDTA, 1 mM NaF, 1% NP40, and 0.1% SDS

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First, gather your materials

So it's like being an archeologist and you're surrounded by chips and pieces of marble, some pots, and you're trying to figure out where they came from, what kind of a civilization produced them, and we're sort of in that situation with the solar system