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After the centrifuge, digestion was performed in 0.05% trypsin-EDTA (T4174, Sigma-Aldrich, MO, USA) for 5 min at 37 C and stopped by adding an equal volume of trypsin inhibitor (T6522, Sigma-Aldrich) in phosphate-buffered saline (PBS)

doi:10.1007/s12325-021-01624-x

While simply adjusting vitamin levels by increasing intake of the foods or supplements you need can help alleviate symptoms, including fatigue, a clinical diagnosis of B12 deficiency is necessary to get the shots

Dan Vu Nguyen, junior, Deans List, College of Arts and Sciences, political science

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