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P.MurphyG.HembryR

Investigators have employed various techniques to assess stochastic gene expression and transcriptional bursting in particular and identify the associated molecular mechanisms: e.g., using single-molecule RNA single molecule fluorescence in situ hybridization (smRNA FISHFISH), using the MS2 green fluorescent protein to illuminate RNA stem loops by epifluorescence or confocal microscopy, and monitoring short-lived protein reporters allow the quantification of transcriptional bursting in particular

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Neuroblastoma cells were maintained in Dulbeccos Modified Eagle Medium (Corning cat