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The peptides were analyzed by data-dependent acquisition (DDA) in positive mode using Orbitrap MS analyzer for precursor scan at 120,000 FWHM from 400 to 2000 m/z and ion-trap MS analyzer for MS/MS scans in top speed mode (3-s cycle time) with dynamic exclusion settings (repeat count one and exclusion duration 15 s)

Peptides cannot bridge a structural gap without tissue continuity

Sample: Fresh capillary blood (fingerstick) or non-anticoagulated venous blood

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