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Ethics declarations Ethics approval The Experimental Animal Center at China Medical University approved all the animal experiments (Approval number: CMU20240820)

The spike in LH And FSH creates a feedback loop which downregulates GnRH receptors causing a decrease in LH and FSH

Separations on the LC-MS were performed on a CAPCELL CORE C18 (2.7 m, 2.1 50 mm, SHISEIDO Co., Ltd., Tokyo, Japan) using a linear gradient of 5100% solvent B over 8 min at a flow rate of 0.4 mL/min