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Furthermore, rv1127c is truncated, encoding only the PPDK N-terminal ATP-binding domain and the central catalytic histidine kinase domain but not the pyruvate/PEP binding domain (Fig

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Blood was collected from the saphenous vein, and blood glucose levels were measured using an ACCU-CHEK Guide Glucose Monitor and Testers (Roche) before each injection and at 10, 20, 30, 60, and 90 min after the glucose injection

Antibodies were detected using Cy2, Cy3, or Cy5conjugated secondary Fab fragment antibodies (Jackson Laboratories) and nuclei stained using Hoechst 33342 (1:1,000, SigmaAldrich)