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Their best improvement is 5 per cent

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Blocking was followed by incubation with the primary antibodies, namely, anti-phospho-p38 MAPK (1:1000, Cell Signalling, 9211, Massachusetts, USA), anti-p38 MAPK (1:1000, Cell Signalling, 9212, Massachusetts, USA), anti-phospho-STAT6 (1:1000, Cell Signalling, 56554, Massachusetts, USA), anti-STAT6 (1:1000, Cell Signalling, 5397, Massachusetts, USA), anti-VEGFA (1:1000, Abcam, ab46154, Cambridge, UK), anti-IL8 (1:1000, Proteintech, 17038-1-AP, Chicago, USA), and anti--actin (1:5000, Proteintech, HRP-60008, Chicago, USA) at 4 C overnight

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Pro-inflammatory cytokines such as IL-1 and TNF produce toxicity through multiple pathways including complement activation, production of arachidonic acid metabolites and ROS formation (Neher et al., 2011)