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An additional office visit will be necessary each year for ongoing therapy or regular intermittent therapy

In some embodiments, the invention encompasses a method of providing muscular therapy by contacting a cell expressing NNMT with a NNMT inhibitor (such as a cation of Formula I or IA, or otherwise disclosed herein) and one or more chemical entities (e.g., nicotinamide riboside, nicotinamide mononucleotide) that increases intracellular NAD+ levels to provide muscular therapy

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Consistently, pull-down assays in HeLa cells showed that endogenous SMC3, SMC1, RAD21, and SA2 interacted with GST-Sororin-CTR, but not with the WA/EK or FA/FA mutants (Fig