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The following includes the key steps to address the hurdles and validate the use of nanotechnology: Preclinical research Nanoparticle-enhanced drug delivery and imaging techniques help in the initial validation, which often begins with in vitro (cell culture) experiments to assess the nanomaterials biological interactions, cytotoxicity, drug release profiles, and targeting abilities, mostly done in preclinical research through in vitro studies and using animal models [122, 123]

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However, its important to know what side effects occur when you take too much

In Project Orbis there are now three types of applications which differ in the timelines of submission and associated regulatory action to FDA and participating partners (regular, modified or written report)