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Single-cell multi-omics analyses revealed significant dynamic heterogeneity in mtDNA mutation accumulation

2.4 Saliva collection Non-stimulated saliva was collected by the spitting method after an overnight rest, always between 8.00 and 9.00 a.m

The same E 1 E 2 E 3 architecture occurs in all three complexes, both in prokaryotes and eukaryotes

For example, active or passive smoking induces oxidative stress and systemic inflammation, impairing ovarian function and insulin sensitivity, thereby exacerbating metabolic and reproductive dysfunction in PCOS (21)

These results were confirmed by Western blot analysis, where protein expression levels were increased according to the marker (Figure 7D)