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Promoter demethylation and chromatin remodeling by green tea polyphenols leads to re-expression of GSTP1 in human prostate cancer cells [J]

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Gene expression levels were normalized to the geometric mean of three housekeeping genes, TATA binding protein (TBP), hypoxanthine phosphoribosyltransferase (HPRT), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and expressed relative to control using the threshold cycle ( C T ) method (19)

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While the palmitic acid and other helpful nutrients from coffee travel to the liver, the coffee stays in the lower colon until it is eliminated and typically does not generate a coffee buzz