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Further studies have shown that HFD can reduce the expression of GLP-1 receptors in the liver, while supplementation with butyrate, a GLP-1 sensitizer, upregulates the levels of GLP-1 receptors in the liver, which stimulates AMPK phosphorylation (69)

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26, 27 GPx activity was slightly but not significantly lower in SHR at 2 weeks of age when compared with WKY rats (Figure 3) and whether the slightly lower GPx activity at 2 weeks of age and afterward was due to the overexpression of CAT or vice versa in this study is uncertain

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Experimental Design of in vitro and in vivo Studies To investigate the effect of IGF-1 on IL-1-induced chondrocytes, cells were plated in a 6-well plate at a density of 510 6 cells/well