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React 0.1 ml protein fraction separated by gel filtration or purified membranes with 5 l of 10 mM DTT for 15 min

Collectively, these findings suggest potential benefits of Mn at low concentrations in mitigating the adverse effects of AGEs on endothelial cells, including the restoration of cell viability, attenuation of oxidative stress, suppression of NF-B-mediated inflammation, and improvement of NOS3 expression and activity

NAC is a thiol (-SH) compound with the ability to directly scavenge hydroxyl radicals

10.1016/j.jlumin.2018.09.015 153 RobertsJ

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