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The concentration of the conjugated antibody was then determined by NanoDrop (Thermo Fisher Scientific) measurement, after which the antibody was diluted with stabilization buffer (Candor Bioscience) to a final concentration of 0.5 mg/mL

Unlike many antioxidants that we get from food and supplements, glutathione is actually produced in the body from three precursor amino acidscysteine, glycine, and glutamine (1)

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With the exception of the poorly active hybrid protein ScGrx8 from yeast 11 , the second cysteine residue of enzymatically active Grx is dispensable for the reduction of glutathionylated substrates and the low-molecular weight model substrate bis(2-hydroxyethyl)disulfide (HEDS) 15,16,17,18,19,20,21,22,23,24,25

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