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Typically, cells were seeded in 100 L RPMI phenol red free media per well in duplicates on a black 96-well plate with clear bottom (Thermo Scientific)

We believe that differences in responses within supposedly homogenous subsets of patients will be extremely informative from the pathogenic point of view and will provide substantial advancement

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All three strains grew equally well following supplementation with isotopically light GSH or [ 13 C 2 , 15 N 1 ]-GSH (S4 and S5 Figs), suggesting that neither cysteine, glycine, nor glutamate were limiting under the tested conditions

A late increase (4872 hours) in the level of Np73 in p53 dom/neg MDD2 breast cancer and normal MCF-10A mammary cells was not found in MCF-7 and MDA-MB-231 cells (Figure 7A)