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Description

Hearts were minced finely in isolation buffer (300 mM sucrose, 10 mM Hepes, 2 mM EGTA, pH 7.2) at 4C, in the presence of 0.5 M type I protease (bovine pancreas) to release mitochondria from the muscle fibers, and then washed in the same buffer in the presence of 1 mg/mL BSA

,Annals of the Rheumatic Diseases 2019 Jun 15;78:1317-8

Step 4: Calculate the Concentration per Unit on an Insulin Syringe

Despite encouraging preclinical findings, clinical data are currently absent, and prioritization of early-phase human trials is necessary before any definitive conclusions can be drawn