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The cleared media was adjusted to a final concentration of 50 mM sodium/potassium phosphate at pH 6.5 and applied to the cation exchange column (Hitrap SP HP, Cytiva)

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Bipolar disorder: A landmark RCT (n=75) found that NAC (1,000 mg twice daily for 24 weeks) produced a significant improvement in depression scores (Montgomery-Asberg Depression Rating Scale) in bipolar disorder as an adjunct to usual treatment (Berk et al., Biol Psychiatry, 2008) [74]