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Cells were maintained in 5% CO 2 at 37 C and grown in DMEM medium containing 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, penicillin (50 U/mL) and streptomycin (100 mg/mL) (All medium and supplements were obtained from GIBCO)

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120 Additionally, the transcription of MT1/2 genes is governed by MTF-1, a zinc finger transcription factor that regulates the expression of metal-responsive genes

Primate studies use single injections whereas human trials often rely on bilateral delivery, making dose extrapolation uncertain

Dr Raghu reports receiving fees for serving as an ad hoc discussant from Bristol Myers Squibb and United Therapeutics, receiving consulting fees from Veracyte, receiving fees for reviewing investigator initiated research grant proposals from Boehringer Ingelheim, providing unpaid consulting services for Biogen, Bellerophon Therapeutics, Blade Therapeutics, FibroGen, Nitto, Roche/Genentech, and Novartis, serving (unpaid) on a data and safety monitoring board for Avalyn, and No other potential conflict of interest relevant to this manuscript