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After centrifugation at 10,000 x g for 10 min at 4 C, the lysate was transferred to 1.5 mL microtubes (Eppendorf, Hamburg, Germany) and stored at 80 C until analysis, but not longer than 1 month

Guanidinoacetate methyltransferase catalyzes the methyl group transfer from S-adenosylmethionine to GAA, yielding creatine (Portocarero and Braun, 2021)

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J Agric Food Chem [Internet]

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