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Seine Plasmakonzentration liegt bei jungen Erwachsenen (etwa 20 Jahre) bei circa 200 ng/ml, sinkt aber bis zum Alter von etwa 60 Jahren auf rund 80 ng/ml

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Post-centrifugation at 350 g for 5 minutes, cells were lysed in 3 ml ACK buffer for 5 minutes, then diluted to 35 ml with RPMI with HEPES buffer and without antibiotics (RPMI-MGIA) and centrifuged again

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