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Description

When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature

Additionally, unique metabolites were identified between the model and bulb groups, implicating three primary metabolic pathways: amino acid metabolism, glucose metabolism, and the flavonoid pathway