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154,155,156,157 Currently, the CRISPR/Cas9 system can enter cells for gene editing in three forms: the Cas9: sgRNA ribonucleoprotein complex, mRNA for Cas9 translation alongside a separate sgRNA, and a DNA plasmid that encodes both the Cas9 protein and sgRNA

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Key Takeaways The gold standard for diagnosing food allergy is an oral food challenge

A study examining b.End.3 cells exposed to high glucose levels in vitro demonstrated a dose-dependent increase in permeability, with increased HIF-1 and decreased expression of the TJ proteins, ZO-1 and occludin (Yan et al., 2012)