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The seeded scaffolds were washed with PBS, fixed in 4% paraformaldehyde for 30 min, and then rinsed to eliminate the PFA

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Vitamin C helps reactivate glutathione when it has been used, allowing it to ensure optimal cellular defense against free radicals

Consistent with the results observed in in vivo experiments, GHK-Cu significantly restored Nrf2 expression in the cytoplasm and reduced it in the nucleus while significantly reducing Keap1 levels in the cytoplasm compared with the CSE-exposed cells without GHK-Cu (Figures 6DF)