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Before experiment conduction, malachite green reagent was prepared freshly by mixing 4.2% (w/v) ammonium molybdate in 4 M HCl and 0.045% (w/v) malachite green in H 2 O in a 1:3 ratio and filtered through a 0.22-m syringe filter

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n=4 per group) were analyzed using a 2DLC-MS workflow and TMTPro isobaric reagents in a SP3 method on a Thermo Lumos tribrid instrument (UC, Davis Proteomics Core Facility)

Mitochondrial membrane potential and glutamate excitotoxicity in cultured cerebellar granule cells