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The cells were lysed in 25 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1% -mercaptoethanol, 10 mM MgCl 2 , 2.1 mM leupeptin, 1.53 mM aprotinin and 50 U of benzonase (Novagen)

Research has shown that in HGSOC with a deficiency of tumor-infiltrating lymphocytes, EZH2 expression in cisplatin-resistant tumors is nearly twice that of normal tumors ( The high intratumoral heterogeneity (ITH) in HGSOC results in diverse molecular characteristics at different stages, consequently affecting its sensitivity and resistance to chemotherapy ( The composition and structure of the tumor microenvironment directly influence drug delivery efficiency