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When multiple probes indexed the expression of the same gene, we selected the probe with the most consistent pattern of regional variation across donors (that is, with the highest differential stability) 75

Construction of expression plasmids for reporter proteins carrying the Azo-tag PCR was performed using the Q5 Hot Start High-Fidelity Polymerase (New England Biolabs) according to the manufacturers protocol

We would like to emphasize that, although both studies were conducted on the same cells, for some aspects cannot be directly compared